The HALO® BIOCLASS peptide columns and phases are specifically designed to offer selectivity, stability, and reproducibility to address complex peptide separations.
Excellent options for both fast and high-resolution UHPLC separations, HALO® delivers high peak capacity and is extremely stable at high temperatures and low pH. For peptide characterization, the optimized 160 Å pore size is ideal for small protein fragments and peptides.
HALO® BIOCLASS peptide columns deliver:
- Optimized 160 Å pore size on a range of chemistries ideal for peptide analysis
- Ideal for both ultrafast and ultra-high-resolution separations of peptides and polypeptides up to 20 kDa
- Approximately 20% higher peak capacity than sub-2 µm non-core columns at comparable back pressure (2 µm)
- Extremely stable at high temperatures and low pH
- Columns of 2.7 and 5 µm can be used in series for ultimate in peak capacity of complex tryptic digest samples
![AMT-BioClass-peptide-white peptide separations](https://halocolumns.com/wp-content/uploads/2021/04/AMT-BioClass-peptide-white.jpg)
Applications
-
Tryptic digests
-
Post-Translational Modifications (PTMs)
-
Variants
-
Polypeptides
Features
- Fast separations
- High peak capacity
- Rugged, reliable performance
- Alternative selectivity with ES-C18, ES-CN, Phenyl-Hexyl
Available Particles
![peptide-160a peptide](https://halocolumns.com/wp-content/uploads/2021/04/peptide-160a.png)
ULTRA FAST PEPTIDE SEPARATIONS
![poresize106A-2um pore size report](https://halocolumns.com/wp-content/uploads/2021/04/poresize106A-2um-1.png)
Due to the Fused-Core® design and excellent mass transferability, ultra fast separations are achievable with HALO® HPLC columns for peptide separation.
![AMT_160A_2_7_RGB 160 Å 2.7 µm molecule](https://halocolumns.com/wp-content/uploads/2020/04/AMT_160A_2_7_RGB.jpg)
BEST USES:
Optimal particle size for LC, UHPLC, and LCMS separations of peptides.
BONDED PHASES:
MAINTAIN HIGH RESOLUTION
![poresize160A-27um glycan analysis](https://halocolumns.com/wp-content/uploads/2021/04/poresize160A-27um-1.png)
HALO® HPLC columns for peptide separation can be increased 2-fold while maintaining high-resolution due to the Fused Core® particle design.
POWERFUL MULTIPLEXING
![base peak mass chromatogram](https://halocolumns.com/wp-content/uploads/2021/04/poresize160A-5um-1.png)
In this example, two 0.2 x 250 mm HALO® 160 Å ES-C18, 5 µm nanoscale columns were connected in series to provide additional resolving power for LC analysis of the tryptic digest of reduced and alkylated trastuzumab.