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The HALO® BIOCLASS peptide columns and phases are specifically designed to offer selectivity, stability, and reproducibility to address complex peptide separations.

Excellent options for both fast and high-resolution UHPLC separations, HALO® delivers high peak capacity and is extremely stable at high temperatures and low pH. For peptide characterization, the optimized 160 Å pore size is ideal for small protein fragments and peptides.

HALO® BIOCLASS peptide columns deliver:

  • Optimized 160 Å pore size on a range of chemistries ideal for peptide analysis
  • Ideal for both ultrafast and ultra-high-resolution separations of peptides and polypeptides up to 20 kDa
  • Approximately 20% higher peak capacity than sub-2 µm non-core columns at comparable back pressure (2 µm)
  • Extremely stable at high temperatures and low pH
  • Columns of 2.7 and 5 µm can be used in series for ultimate in peak capacity of complex tryptic digest samples
peptide separations

Applications

  • Tryptic digests
  • Post-Translational Modifications (PTMs)
  • Variants
  • Polypeptides

Features

  • Fast separations
  • High peak capacity
  • Rugged, reliable performance
  • Alternative selectivity with ES-C18, ES-CN, Phenyl-Hexyl

Available Particles

peptide

BEST USES

Fast, ultra-high-resolution UHPLC separations of peptides

BONDED PHASES

ES-C18

ULTRA FAST PEPTIDE SEPARATIONS

pore size report

Due to the Fused-Core® design and excellent mass transferability, ultra fast separations are achievable with HALO® HPLC columns for peptide separation.

160 Å 2.7 µm molecule

BEST USES:

Optimal particle size for LC, UHPLC, and LCMS separations of peptides.

BONDED PHASES:

ES-C18, Phenyl-Hexyl, ES-CN

MAINTAIN HIGH RESOLUTION

glycan analysis

HALO® HPLC columns for peptide separation can be increased 2-fold while maintaining high-resolution due to the Fused Core® particle design.

peptide

BEST USES

Multiplexing in series for added peak capacity, lowest back pressure delivery.

BONDED PHASES

ES-C18, ES-CN

POWERFUL MULTIPLEXING

Base Peak Mass Chromatogram

In this example, two 0.2 x 250 mm HALO® 160 Å ES-C18, 5 µm nanoscale columns were connected in series to provide additional resolving power for LC analysis of the tryptic digest of reduced and alkylated trastuzumab.

Featured Documents

Application Notes

Technical Literature

Catalog

Halo Bioclass For Glycan Analysis, Protein Separation And Peptide Separation

Guidebook

Halo Columns Bioanalysis Solutions Guidebook
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