HALO® Technical Resources | HPLC Information & Applications

New Dimension in Chromatography - Halo^® 1.5

Application Notes

Conference Papers

Product Literature

Technical Documents

Improved LC Separations of Nucleic Acids Using Large Pore Superficially Porous Particles
Conference Paper

Novel High pH Stable SPP Columns for Enhanced LC and LCMS Separations of Basic Compounds
Conference Paper

High Quality Reproducibility in SPP HPLC Product Manufacturing
Conference Paper

Current LC/MS Approaches for PFAS Analysis with Ultrashort and Long Chain Mixtures
Conference Paper

Improved Bioseparations with a Novel Charged Surface Superficially Porous Silica Column
Conference Paper

HPLC Approaches to Improve Peak Shape for Basic Analytes
Conference Paper

LC/MS Approaches for Analysis of PFAS Short and Long Chain Mixtures
Conference Paper

Current LC/MS Approaches for PFAS Analysis with Short and Long Chain Mixtures
Conference Paper

Improving Chromatography for Basic Analytes Using Positive Charge Surface Material
Conference Paper

Method Development Approach to Separating Oligonucleotides Under UV and MS Detection
Conference Paper

Enhancing LC and LC-MS Separations of Basic Compounds with Novel High pH Stable SPP Columns
Conference Paper

New Column Technology for Improved Oligonucleotide Separations
Conference Paper

Improving Short Chain PFAS Analysis Using Reversed Phase Conditions
Conference Paper

Evaluation of Positively Charged Surface Stationary Phases for Improved Chromatographic Separations of Basic Analytes in Small Molecules and Peptides
Conference Paper

Method Development Approach to Separating Oligonucleotides with Superficially Porous Particle Columns
Conference Paper

Evaluation of Positively Charged Surface Phenyl-Hexyl Stationary Phase for Separation of Basic Small Molecule Drugs
Conference Paper

Demonstration of the Utility of a 1.5 mm ID UHPLC column for Pesticide Analysis Using the Multi-Analyte Method
Conference Paper

Evaluation of Positively Charged Surface Phenyl-Hexyl Stationary Phase for Separation of Basic Small Molecule Drugs
Conference Paper

Charged-Surface Stationary Phase Improves Peptide Separations in Low Ionic Strength Mobile Phases
Conference Paper

HPLC Separation Improvement for Short Chain PFAS
Conference Paper

Positively Charged Surface Chemistry for Biological Separations
Conference Paper

Small Molecule HPLC Method Optimization using Positive Charge Surface
Conference Paper

Drugs of Abuse and Metabolite Screening Using High Efficiency LC Columns
Conference Paper

Impact of Using 1.5 mm ID Column and Various Stationary Phases on LCMS Pesticide Screening Analysis
Conference Paper

Modernizing USP Methods According to <621> with Superficially Porous Particle Columns
Conference Paper

Increased Efficiency of Protein and Peptide Separations by Varying Particle Size, Column Dimension, and Pore Size of Superficially Porous Particle Columns
Conference Paper

Small Molecule HPLC Method Optimization Using an Acidic, Basic, and Neutral Panel and Superficially Porous Particles
Conference Paper

RPLC-MS analytical method for the separation of all hydroxyproline isomers and application to different collagen hydrolysates
Conference Paper

Implementing 1.5 mm inner diameter columns into LC-MS bottom-up proteomic workflows
Conference Paper

Increasing Efficiency of Peptide Separations by Decreasing Particle Size and Column Dimension
Conference Paper

A Novel Screening Approach for Comparing LC-MS Reversed-Phase and HILIC Methods for Separations in Biological Matrices Using Amino Acid Examples
Conference Paper

Opportunities for Developing Improved Separation Methods with Modern UHPLC Instruments
Conference Paper

Comparison of LC-MS Pesticide Screening Results Using Different Reversed-Phase Superficially Porous Columns
Conference Paper

Cannabinoid Separation Using LPH-C18
Conference Paper

Optimizing Older UHPLC Systems for Use with New, Smaller ID Columns
Conference Paper

A Simple Method for Predicting HPLC Selectivity in Ternary Reversed-Phase Solvent Systems
Conference Paper

Monoclonal Antibody Analysis with Compact Capillary LC Instrumentation
Conference Paper

Enhanced Sensitivity for Peptide and Protein Applications Using the 1.5mm ID Column
Conference Paper

LPH-C18: A C18 Column Alternative
Conference Paper

Exploring the Improvements Enabled by 1.5 mm ID UHPLC SPP Columns
Conference Paper

Implementing 1.5 mm ID Columns into Analytical Workflows
Conference Paper

Enhanced Sensitivity for Intact Monoclonal Antibody Analysis via LCMS using a Novel UHPLC Column Design
Conference Paper

New 1.5 MM UHPLC Columns Enable Robust Separations with Increased Sensitivity and Solvent Savings
Conference Paper

Separation of Short and Long Chain Oligonucleotides through the Use of a Large Pore SPP Column
Conference Paper

Development of a Sensitive, High‐Resolution LCMS HILIC Method of the Oxidation of NIST mAb as an Alternate to Reverse Phase
Conference Paper

LC/MS HILIC Analysis for Glycosylation and Deamidation of Peptide and mAbs
Conference Paper

LC-MS Method Development and Column Screening for Pharmaceutical and Personal Care Products in the Environment
Conference Paper

Selection of HPLC and LC/MS Conditions for the Separation of Polar Compounds and Protein Modifications
Conference Paper

Rapid PFAS Analysis Using Fused-Core Technology
Conference Paper

Fast Screening of Oligo and Poly-Saccharides in Beer
Conference Paper

Optimizing Particle Pores for HPLC Separation of Verapamil and other Mid-Size Molecule
Conference Paper

Screening Mycotoxins on Sorghum Grain viaSuperficially Porous Particle Columns
Conference Paper

Applications of SPPS in Environmental Analysis
Conference Paper

AQ-C18-Official Pittcon 2019 Poster-1
Conference Paper

C30-Official Pittcon 2019 Poster-2
Conference Paper

Superficially Porous Particles with C30 Stationary Phase for High Resolution Separations
Conference Paper

The Importance of Superficially Porous Particles in Modernizing HPLC Methods
Conference Paper

Characterization of the Activity and Kinetics of Guanine Deaminase
Conference Paper

High Resolution Separation for Detailed LC/MS Analysis of mAb Disulfide Variants
Conference Paper

Advantages of Superficially Porous Particles (SPP) for Faster HPLC and UHPLC Separations
Conference Paper

A Superficially Porous Particle (SPP) C30 Phase Designed for High Speed HPLC Applications
Conference Paper

Wide Pore Superficially Porous Particles with Various Bonded Phases For High Resolution Protein Chromatography
Conference Paper

Probes-Official Pittcon 2019 Poster-3
Conference Paper

Selectivity Manipulation for LC/MS Analysis of Protein Variants
Conference Paper

LC/MS Method for Analysis of Guanine Deaminase: Activity, Kinetics and Effects of Inhibitors
Conference Paper

EAS 2018 All You Wanted to Know about Method Development and Transfer but Were Afraid to Ask
Conference Paper

EAS 2018 New RP SPP Columns with Alternate Selectivity
Conference Paper

EAS 2018 Optimizing HPLC Separation Performance for Mid-Size Molecules
Conference Paper

EAS 2018 Wide Pore SPP Bonded Phase
Conference Paper

High Performance Separations using 100% Aqueous Mobile Phase Compatible Superficially Porous Particle Columns Coupled with Mass Spectrometry
Conference Paper

A Diphenyl Bonded Phase On Wide Pore Superficially Porous Particles For Efficient Separations of Proteins
Conference Paper

LC/MS Method for the Analysis of Guanine Deaminase
Conference Paper

One Size Does Not Fit All: Exploring the Relationship Between Pore Size and Separation Efficiency
Conference Paper

Pittcon 2018 New Superficially Porous ParticleColumn Selectivity Choices for Improved Separations
Conference Paper

Pittcon 2018 – Column Pore Size – An Underutilized Variable for Optimizing HPLC Separation
Conference Paper

2017 ASMS ThP 601 High Efficiency Wide Pore SPPs for LC-MS of Larger Particles
Conference Paper

How to Avoid Mismatch Between Solutes and Column Pores for Optimum HPLC Performance
Conference Paper

Superficially Porous Particles with Polar C18 Selectivity
Conference Paper

An Effective Method Development Strategy Using FUSED-CORE Columns
Conference Paper

HPLC 2017 Improving Biomolecule Separations with Superficially Porous Particles of Silica
Conference Paper

Improve HPLC Separations by Carefully Matching Column Pore-Size to Solute-Size MCF 2017
Conference Paper

ISPPP 2017 Comparison of Phenyl- and C18 Bonded Phases with Peptide Mixtures
Conference Paper

ISPPP 2017 High Efficiency Wide Pore SPPs for LC-MS of Larger Proteins
Conference Paper

Pittcon 2017 – Developing Methods When C18 Does Not Work
Conference Paper

EAS 2016 Impact of Pore Exclusion on RP-HPLC Column Performance
Conference Paper

EAS 2016 Peptide Mapping Analyses Using Different Mobile Phase Additives
Conference Paper

ISPPP 2016 LC-MS of mAb Structure Utilizing HALO BioClass Fused-Core Particles
Conference Paper

EAS 2015 LCMS Analysis of Monoclonal Antibody Structure Utilizing HALO BioClass Fused-Core Particles Multilevel Analysis for Proteins and Glycovariants
Conference Paper

HPLC 2015 Performance Features of 2 um Superficially Porous Particle Columns
Conference Paper

ISPPP 2015 Rapid and Specific HILIC Method to Assay Guanine Deaminase and Application to Characterize the Brain Enzyme
Conference Paper

ASMS 2015 – Prediction of Peptide RT in HILIC Poster
Conference Paper

Pittcon 2015 – Alternatives to Sub 2-micron UHPLC Columns
Conference Paper

2017 ASMS ThP 539 HILIC LC-MS Analytical Approach for ID of Protein Deamidation and Isomerization Modifications
Conference Paper

HPLC_2016_Improving Protein Separations
Conference Paper

ASMS 2016 High Resolution Separations for Protein LC MS
Conference Paper

ASMS 2015 Utilization of an Isotopically Labelled Glycoprotein Internal Standard to Enable Comparison of Glycan Quantitation
Conference Paper

ASMS 2015 Improving Protein LC-MS Analysis
Conference Paper

Characterization of Guanine Deaminase
Conference Paper