The New HALO® PCS – Positively Exceptional Performance
Built upon proven Fused-Core® technology for speed and efficiency, the HALO® PCS column products are positively charged surface chemistries designed to deliver improved peak shapes for basic compounds observed with standard C18 and Phenyl-Hexyl chemistries. Ideal for use with low ionic strength mobile phases, HALO® PCS maintains peak symmetry at higher loading capacities and provides alternate selectivities from other C18 and Phenyl-Hexyl bonded phases. Available in both a 90 Å and 160 Å pore size for small molecule and peptide analysis. The columns are optimized to deliver performance for reproducible, high efficiency LC and LCMS separations.
Delivering More Performance
![check-mark check-mark](https://halocolumns.com/wp-content/uploads/2022/01/check-mark-20x20.jpg)
Excellent Peak Shape and Increased Loading Capacity for bases
![check-mark check-mark](https://halocolumns.com/wp-content/uploads/2022/01/check-mark-20x20.jpg)
UHPLC and LCMS Compatible
![check-mark check-mark](https://halocolumns.com/wp-content/uploads/2022/01/check-mark-20x20.jpg)
Built upon Fused-Core® Technology for fast, efficient and reliable separations
Three NEW Phases to Choose From:
![SM-RP-HALO 90 Å PCS C18](https://halocolumns.com/wp-content/uploads/2024/01/SM-RP-HALO-90-A-PCS-C18.jpg)
- 2.7 µm particle size, 90 Å pore size optimized for small molecules
- Alternate L1 Selectivity
- Basic pharmaceutical drugs, environmental contaminants, drugs of abuse
![SM-RP-HALO 90 Å PCS PHENYL HEXYL-Updated](https://halocolumns.com/wp-content/uploads/2024/04/SM-RP-HALO-90-A-PCS-PHENYL-HEXYL-Updated.jpg)
- 2.7 µm particle size, 90 Å pore size optimized for small molecules
- Alternate L11 Selectivity
- Basic pharmaceutical drugs, environmental contaminants, drugs of abuse
![B-PE-HALO 160 Å PCS C18](https://halocolumns.com/wp-content/uploads/2024/01/B-PE-HALO-160-A-PCS-C18.jpg)
- 2.7 um particle size, 160 Å pore size optimized for peptide separations
- Alternate L1 Selectivity
- Peptide mapping and detection, impurity analysis
HALO® Advantage!
- A mix of four antidepressants is separated using the HALO 90 Å PCS C18 column.
- The positive charged surface (PCS) stationary phase is ideal for basic analytes when using low ionic strength mobile phases such as formic acid.
- Improved tailing factor and efficiency are observed when compared to a traditional (uncharged) C18 stationary phase.
Separation Using HALO® PCS C18 Compared to C18
![Performance](https://halocolumns.com/wp-content/uploads/2024/03/Performance-1.jpg)
*see brochure for full test conditions
Panel of Antidepressants Screened with 900 Injections
Demonstrating the Excellent Stability of HALO® PCS C18
![Stability](https://halocolumns.com/wp-content/uploads/2024/03/Stability.png)
*see brochure for full test conditions
- A HALO® Peptide PCS C18 outperforms a standard peptide C18 column under formic acid conditions due to its positive charge surface, allowing for improved peak shape and resolution for peptides.
- HALO Peptide also allows for a higher sample load on column for basic analytes and could potentially help pull apart closely retained impurities as seen in the graphs.
Peptide Loading Studies
![HALO® PCS C18 vs Standard Peptide C18 Chromatogram](https://halocolumns.com/wp-content/uploads/2024/03/HALO®-PCS-C18-vs-Standard-Peptide-C18-Chromatogram.png)
*see brochure for full test conditions
NEW Featured Documents
taking advantage of fused-core® technology
Visit our solutions pages to learn more about the best uses and benefits of Fused-Core® Technology.
![sm-circle hplc columns for small molecule analysis](https://halocolumns.com/wp-content/uploads/2021/04/sm-circle-300x300.png)
From an ultra-fast separation of a few compounds to a more complex natural product sample containing hundreds of analytes, HALO® columns offer an optimized solution for separation challenges.
![bioclass-circle lc column for biopharma solutions](https://halocolumns.com/wp-content/uploads/2021/04/bioclass-circle-300x300.png)
Biopharmaceuticals are inherently more complex than small molecules. HALO® BioClass columns have been specifically designed to accomplish these bio-separation goals with a simplified and more effective solution.
![enviroclass-circle hplc environmental applications](https://halocolumns.com/wp-content/uploads/2021/04/enviroclass-circle-300x300.png)
Solutions for other persistent, high environmental-impact contamination agents such as pesticides, mycotoxins, herbicides, and more all based upon HALO’s 15 years of innovative and trusted technology.