ASMS 2026
START YOUR MORNING WITH COFFEE and OLIGOs!
SEMINAR TOPIC:
Mastering The Art of Oligonucleotide Analysis via LCMS Using The HALO 1000 Å OLIGO C18 HPLC Column
Date: Wednesday, June 3, 2026
Location: Room 1A
Time: 7:00am- 8:00am (light breakfast will be provided)
Speaker: Dr. Josh McBee
Oligonucleotide-based therapeutics are a rapidly growing area that present unique challenges for analytical analysis. LC/MS has excellent utility for performing critical quality attribute (CQA) testing but can present several novel hurdles for MS users accustomed to performing routine small molecule, protein, or peptide analysis. Their large size, hydrophilic nature and separation challenges require unique approaches.
This seminar will demonstrate how to build an ion pair/reversed phase (IP/RP) LC/MS workflow using our novel HALO 1000Å OLIGO C18 HPLC columns. We will discuss proper sample prep and handling, ion pairing reagent selection and procedures for making IP buffers, and how to minimize adduction risk when performing MS analysis.
We will show several applications including siRNA impurity analysis, mRNA mapping and 5’-cap CQA analysis, and analysis of sgRNA’s for CRISPR applications.
We hope this seminar will provide MS users with confidence in approaching oligonucleotide analysis and a framework for proper method development.
About the Speaker
JOSHUA MCBEE, PHD
Joshua McBee has a Masters in physical chemistry and earned his Ph.D. from the University of Washington in BioOrganic Chemistry. His postdoctoral work was conducted in the Department of Neurological Surgery at the University of Washington and with Dr. Ruedi Aebersold at the Institute for Systems Biology, focusing on novel approaches to untargeted and quantitative targeted proteomics and metabolomics.
Josh has worked for Accelerator Ventures on early stage biopharma startups, Agilent Technologies in a variety of roles including R&D and service field support, sales and marketing and more. Prior to joining AMT, he worked at MOBILIon Systems, a startup focused on high-resolution ion mobility technologies.
Josh role at AMT is focused on his Mass Spectrometry subject matter expertise and bio-separations including protein, peptide, and oligonucleotide separations. Currently, his primary interests focus on educating customers on the complexities of oligonucleotide separations including siRNA’s, guide RNAs, and mRNA characterization workflows.