HALO® BIOCLASS Oligonucleotide columns are designed with the foundational properties of Fused-Core® technology for high-efficiency, high-speed separations and optimized for the needs of oligonucleotide analysis. With the addition of a new 1000 Å pore-size option—offering enhanced performance for larger, more complex oligos—HALO® now provides an OLIGO solution that spans the full range of separation challenges. Closely related impurities and recognition of failure sequences are easily achieved with the HALO® OLIGO columns.
The HALO® OLIGO C18 incorporates surface modified organo-silane technology for alkaline resistance, resulting in excellent stability under elevated pH operating conditions common in oligonucleotide separation methods. The inert column hardware also addresses absorptivity concerns so the highest recoveries can be achieved.
HALO® BIOCLASS OLIGO COLUMNS DELIVER:
- 120 Å pore size, oligomers up to 60 bases in length
- 1000 Å pore size for high efficiency separation of oligomers up to 100+ bases in length
- High pH and temperature stability, designed for conditions suited for oligonucleotide separations
- UHPLC and mass spectrometry compatible stationary phase
- Surface passivated column hardware to reduce adsorption of oligonucleotides
Performance at a Glance
Rapid Separation of Oligonucleotide Ladder
This example demonstrates the resolution of an Oligonucleotide Standard mixture (10- to 60-mer ssDNA) using triethylammonium acetate (TEAA) with absorbance detection and gradient elution with acetonitrile. This fast separation (less than 3.5 minutes) illustrates the excellent peak shape, and high resolution of oligonucleotides of up to 60 oligonucleotides in length using Advanced Material Technology’s Fused-Core® technology in the 2.1 x 50 mm HALO® OLIGO C18 column.
Excellent Lot-To-Lot Reproducibility
An oligonucleotide ladder of mixed sequence and length is separated on the HALO® OLIGO C18 and a competitor oligonucleotide column under high pH conditions. The oligomers of 20 base length AND higher begin to tail significantly on the competitor column. The same oligomers show no tailing on the HALO® column demonstrating the chromatographic efficiency and speed of Fused-Core®. Note: Tailing of the competitor column could represent poor column loading, however, both the HALO® and competitor columns were QC tested and passed prior to analysis.
Featured Documents
Application Notes
1000 Å OLIGO C18 Brochure
120 Å OLIGO C18 Brochure
SHOP HALO® OLIGO
