Could HILIC Be the Answer?

HILIC continues to gain popularity as more highly polar and ionizable compounds are developed but the mention of moving from the beloved reversed-phase separation mode to HILIC can ignite anxiety in many a method development scientist.  But what do you do when many polar compounds are not well retained under reversed-phase conditions?  Utilizing Hydrophilic Interaction Liquid Chromatography (HILIC) as a mode of chromatography is very beneficial for separations involving very polar analytes. However, some labs consider HILIC as a last resort method and would prefer to avoid its use. This white paper aims to dispel the myths around HILIC and provide options for those struggling with methods for polar analytes, such as metabolites, basic drugs, and carbohydrates.

Key Learning Objectives:

• Understand the advantages of using HILIC for polar analytes
• Learn tips and tricks to successfully develop HILIC methods
• Review example HILIC applications and how Fused-Core® technology benefits them