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New Dimension in Chromatography - Halo® 1.5

HALO® HAPPENINGS

Improved LC and LC-MS Separations of Oligonucleotides Using Superficially Porous Particles

Registration link: https://register.gotowebinar.com/register/7583205701269497693 

When: August 13th, 2025 | 12:00 PM – 1:00 PM EDT

Speaker: Peter Pellegrinelli, Applications Specialist, Advanced Materials Technology, Inc.

Event Overview:

Join us to learn about a new phase chemistry from Advanced Materials Technology to improve LC and LCMS separations of oligonucleotides. With the release of the new HALO® OLIGO C18 stationary phase, chromatographers have a new tool for oligonucleotide separations in addition to realizing the benefits of Fused-Core® particle technology for high speed, high efficiency separations. These new columns are designed for both ssDNA and ssRNA separations up to 60 bases in length. Several applications will be shown to demonstrate:

  • Separations of oligonucleotides ranging from 10 bases in length up to 100 bases in length
  • N -1 separations/Impurity analysis of a 15 base oligonucleotide
  • The advantage of the Fused-Core® particle technology improving oligo separations compared to other column materials

Key Learning Objectives:

  • Discuss the compatibility of the HALO® OLIGO C18 column with ion-pairing reagents, elevated temperature methods, and both UV and MS detection platforms.
  • Explain how HALO® OLIGO C18 supports impurity profiling of oligonucleotides by resolving sequence variants such as N-1, deletion products, and other process-related impurities.
  • Design effective LC method parameters (e.g., mobile phase, temperature, gradient) for oligonucleotide separations using HALO® OLIGO C18 based on sequence length and desired resolution.
  • Assess the benefits of Fused-Core® particle technology for enhancing peak resolution, reducing analysis time, and improving method robustness in high-throughput oligonucleotide labs.

Who Should Attend:

  • Chromatographers and LC/MS method developers who are interested in improving their separations for oligonucleotides as well as chromatographers new to oligonucleotides.

 

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