The HALO® BIOCLASS peptide columns and phases are specifically designed to offer selectivity, stability, and reproducibility to address complex peptide separations.
Excellent options for both fast and high-resolution UHPLC separations, HALO® delivers high peak capacity and is extremely stable at high temperatures and low pH. For peptide characterization, the optimized 160 Å pore size is ideal for small protein fragments and peptides.
HALO® BIOCLASS peptide columns deliver:
- Optimized 160 Å pore size on a range of chemistries ideal for peptide analysis
- Ideal for both ultrafast and ultra-high-resolution separations of peptides and polypeptides up to 20 kDa
- Approximately 20% higher peak capacity than sub-2 µm non-core columns at comparable back pressure (2 µm)
- Extremely stable at high temperatures and low pH
- Columns of 2.7 and 5 µm can be used in series for ultimate in peak capacity of complex tryptic digest samples
Applications
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Tryptic digests
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Post-Translational Modifications (PTMs)
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Variants
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Polypeptides
Features
- Fast separations
- High peak capacity
- Rugged, reliable performance
- Alternative selectivity with ES-C18, ES-CN, Phenyl-Hexyl
Available Particles
ULTRA FAST PEPTIDE SEPARATIONS
Due to the Fused-Core® design and excellent mass transferability, ultra fast separations are achievable with HALO® HPLC columns for peptide separation.
BEST USES:
Optimal particle size for LC, UHPLC, and LCMS separations of peptides.
BONDED PHASES:
MAINTAIN HIGH RESOLUTION
HALO® HPLC columns for peptide separation can be increased 2-fold while maintaining high-resolution due to the Fused Core® particle design.
POWERFUL MULTIPLEXING
In this example, two 0.2 x 250 mm HALO® 160 Å ES-C18, 5 µm nanoscale columns were connected in series to provide additional resolving power for LC analysis of the tryptic digest of reduced and alkylated trastuzumab.
Featured Documents
Application Notes
Technical Literature
Catalog
Guidebook
