Improving Small Molecule Analysis: Increasing the Sensitivity While Reducing Solvent Consumption by Using 1.5 mm ID Columns
AUTHOR: Stephanie Schuster, Ph.D., Senior Technical Support Scientist ABSTRACT The new HALO® 1.5 mm ID columns…
Amino acid analysis (AAA) is an important technique for determining the quantity of amino acids in a sample. In the biopharmaceutical industry, amino acid analysis has become an important tool in quantifying amino acid content in both feed and spent media during upstream fermentation, enabling efficient monitoring of the cell culture process.
This work describes the use of the HALO® penta-hydroxy based column (Advanced Materials Technology) for the HILIC separation of underivatized amino acids. The column is a bonded silica phase which contains a highly polar ligand with five hydroxyl groups tethered via novel proprietary linkage chemistry to Fused-Core® silica particles. The Fused-Core® particles provide higher efficiencies with improved resolution and lower detection limits, lower backpressure, as well as excellent reproducibility and lot-to-lot robustness. To demonstrate the quantitative application, the HILIC separation method is coupled to a single quadrupole (QDa) mass spectrometer for mass detection analysis.
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