Did you see this month’s newsletter? This month’s HALO® Hint explores the chromatographic impact of hydrophobic and steric modifications in siRNAs, including why these changes matter when optimizing siRNA analysis, separation performance, retention, and selectivity. Read the full feature below.…
AUTHOR: Stephanie Schuster, PhD, Senior Technical Support Scientist, Advanced Materials Technology 20 Years of High Quality Fused-Core® Column Manufacturing INTRODUCTION Since our founding in 2005, Advanced Materials Technology has been focused on one mission – Improving the way the sample…
AUTHOR: Conner McHale, Technical Support Specialist, Advanced Materials Technology INTRODUCTION to PFAS ANALYTICAL METHODS Per- and polyfluoroalkyl substances (PFAS) are a group of chemicals used to make fluoropolymer coatings and products that resist heat, oil, stains, grease, and water. These…
The goal for chromatographic separations is to achieve well resolved peaks with adequate retention and demonstrate gaussian peak shapes, so the compounds being separated can be assessed accurately both qualitatively and quantitatively. Peak shape is one of the most common…
Introduction A revolutionary innovation in liquid chromatography occurred in 2006 when Advanced Materials Technology introduced the first sub-3-µm superficially porous particles (SPP) and columns designed for HPLC and UHPLC of small molecules such as pharmaceuticals, industrial chemicals, and the like.…
AUTHOR: Andrew Harron Ph.D., Application Scientist MARKET SEGMENT: BIOCLASS ABSTRACT Decreasing the internal diameter of a column can increase detector response, lower solvent consumption, and bring multiple benefits to the overall analysis workflow. This has fueled the development of capillary…
Author: Dr. Stephanie Schuster, Senior Technical Support Scientist from Advanced Materials Technology For more information, please contact: support@advanced-materials-tech.com INTRODUCTION The guidelines in United States Pharmacopeia (USP) Chapter <621> were recently updated to allow changes to particle size and column dimensions…
Using 1.5 mm ID Columns for Solvent Reduction While Increasing Sensitivity Originally, HPLC methods were developed using 4.6 mm ID columns and greater. At the time, the separations were acceptable, and not much emphasis was placed on either reducing solvent…
AUTHOR: Stephanie Schuster, Ph.D., Senior Technical Support Scientist ABSTRACT The new HALO® 1.5 mm ID columns offer an alternative to analytical and narrow bore columns for greater separation performance with the benefits of increased sensitivity, reduced solvent consumption/reduced waste disposal costs,…
Introduction High-pressure liquid chromatography (HPLC) is a benchmark technique used in laboratories across the world. However, even experienced analysts that are familiar with HPLC concepts and principles can fall victim to chromatographic pitfalls that arise. Some common issues include misshapen…
