The goal for chromatographic separations is to achieve well resolved peaks with adequate retention and demonstrate gaussian peak shapes, so the compounds being separated can be assessed accurately both qualitatively and quantitatively. Peak shape is one of the most common…
AUTHOR: Stephanie Schuster, Ph.D., Senior Technical Support Scientist ABSTRACT The new HALO® 1.5 mm ID columns offer an alternative to analytical and narrow bore columns for greater separation performance with the benefits of increased sensitivity, reduced solvent consumption/reduced waste disposal costs,…
Introduction High-pressure liquid chromatography (HPLC) is a benchmark technique used in laboratories across the world. However, even experienced analysts that are familiar with HPLC concepts and principles can fall victim to chromatographic pitfalls that arise. Some common issues include misshapen…
THE POSITIVES OF PCS The new HALO®PCS C18 phase offers an alternative to chromatographic separations under acidic conditions. Many chromatographers are familiar with the difficulties of separating basic compounds. These compounds are troublesome and usually elute from the column with…
Amino acid analysis (AAA) is an important technique for determining the quantity of amino acids in a sample. In the biopharmaceutical industry, amino acid analysis has become an important tool in quantifying amino acid content in both feed and spent…
The guidelines in USP Chapter <621> were recently updated to allow changes to gradient methods in terms of particle size and column dimensions with verification instead of revalidation. This change enables laboratories to benefit from modern column particle sizes and…
AUTHOR: Conner McHale, Technical Support Specialist and Taylor Harmon, Ph.D., R&D Scientist ABSTRACT Metabolomic analyses are utilized in various avenues of clinical research, including drug discovery, disease characterization, and pharmacodynamic evaluation. Metabolites are often studied via liquid chromatography-mass spectrometry (LC-MS)…
AUTHOR: Peter Pellegrinelli, M.S., Applications Specialist ABSTRACT Vitamins are a crucial nutrient to humans and animals alike. In order for people to live healthy lives they need the right nutrients to enable these healthy conditions. Today, people are more conscious…
