HALO® Literature at a Glance | HALO® Columns for Chromatography Separations

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Application Notes

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Technical Documents

Advantages of HALO® OLIGO C18
Application Note

HALO® OLIGO C18 Lot to Lot Reproducibility
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Elevating pH to Improve a Drugs of Abuse Separation
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Elevating pH to Improve a Drugs of Abuse Separation
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Oligonucleotide Performance Mix on OLIGO C18
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Separation of Oligonucleotide Ladder Via LCMS Using HALO® OLIGO C18
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Oligonucleotide Ladder Via UV Detection
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Omeprazole Improvements on HALO Elevate C18
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HALO Elevate C18 Lot-to-Lot Reproducibility
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Omeprazole Analysis Using HALO Elevate C18
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High Temperature pH Amitriptyline Stability using HALO Elevate
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Nicotine Analysis using HALO Elevate C18
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Separation of Antidepressants on HALO® Elevate
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Separation of Tetracyclines Using HALO 90 A PCS Phenyl Hexyl
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Impact of PCS on the Separation of Glyphosate and AMPA
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Separation of Benzodiazepines and Neutral Molecules using HALO PCS
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Separation of Antidepressants Using HALO PCS C18 Compared to C18
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Quick Separation of Allelopathic Chemicals on HALO®C18
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Water-Soluble Vitamins Separated on HALO® Penta-HILIC
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LCMS Analysis of Drugs and Metabolites on HALO® Biphenyl
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Separation of Amino Acids by HILIC_Single Quadrupole Mass Detection
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Amino Acid Analysis in Biological Matrices Using Reversed Phase and HILIC Mode
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Separation of 19 Cannabinoids using HALO® LPH-C18
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Rapid Separation of Reduced Trastuzumab on HALO 1000 A Diphenyl
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Increased Peak Capacity of Trastuzumab Tryptic Digest on PCS C18
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LCMS-Analysis-Oregon-Pesticide-Mix-HALO-AQC18
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LC-MS Analysis of the Oregon Pesticide Mix on HALO® PCS C18
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Separation of Reserpine on HALO®PCS C18
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Separation of Amine Based Medications on HALO® PCS C18
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Local Anesthetics Separated Using HALO® PCS C18
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Separation of Insulin Variants Using the HALO 160 Å PCS C18
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Separation of Antidepressants Using HALO 90 Å PCS C18
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Demonstration of Increased Sensitivity with Reduced Trast Using a 1.5 mm ID Column Folder
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HALO 160 Å PCS C18 Loading Studies
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HALO 160 Å PCS C18 vs. C18 Peptide Panel
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HALO 160 Å PCS C18 Rapid Peptide Separation
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HALO 90 Å PCS C18 Compared to Competitor SPP Charged Surface Phase
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Beta Blockers Separation on HALO® PCS C18
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Pharmaceutical and Personal Care Products Column Screening
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RP-Amide Stability of Naphthalene
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Isocratic Separation of Phenyl Ureas on HALO® ES-CN
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Optimization of Tocochromanols Separation Using Supercritical Fluid Chromatography and HALO®Biphenyl
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Analysis of Spinach
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USP Itraconazole System Suitability Comparison
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Optimization of Rivaroxaban USP Monograph using Superficially Porous Particle Technology
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A Reversed Phase Separation of Polar Metabolites Using the HALO® 1.5 mm ID AQ-C18 Column
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LCMS Profiling of Olive Oils Using the HALO® C30 Column
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PFAS in Soil Using EPA Method 1633
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Optimization of USP Monograph for Itraconazole
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Trastuzumab Digest Sensitivity Improvement Using a 2µm Particle on HALO®1.5 mm ID
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Separation of Polysaccharide Ladder on the HALO®1.5 mm ID Penta-HILIC Column
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Separation of Local Anesthetics using the HALO®1.5 mm ID Penta-HILIC Column
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Water-Soluble Vitamin Analysis using the HALO®1.5 mm ID AQ-C18 Column
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HILIC Mode Separation of Polar Metabolites Using the Novel HALO®1.5 mm ID Penta HILIC Column
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Improved Signal Intensity for Trastuzumab Peptides Using HALO 1.5 mm ID Column
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Nicotine Metabolite Comparison of HALO 1.5 mm to 2.1 mm ID Column using HILIC Separation Mode
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11 Cannabinoid Separation Using HALO LPH-C18
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6 Cannabinoid Separation using HALO LPH-C18
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Analysis of Sugars in Juice using HALO® Penta HILIC
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Analysis of 27 PFAS Compounds Using a Modified 1633 Method
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Analysis of Eugenol in Clove Oil on HALO® 5 μm C18
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Oligonucleotide Ladder Separation on HALO 1000A ES-C18
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Separation of Fat Soluble Vitamins in Immune Support
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Increased Sensitivity of Vitamin D Metabolites Using LCMS
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Options for Optimizing the USP Monograph for Estradiol
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Impact of Column ID on Sensitivity for Peptides and Proteins
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Impact of Column ID Sensitivity for Small Molecule Pharmaceuticals
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Separation of Anthocyanins in Blueberries
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Separation of 18 Cannabinoids using HALO LPH-C18
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Separation of Common Catechins and Caffeine Found in Tea via LC-UV
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LC-MS Separation of Common Catechins Found in Tea
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Pesticide Screening of Barley: HALO 90 Å LPH-C18
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Separation of Polyphenols in Wine
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Low pH Stability with HALO® LPH-C18
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Separation of EPA 610 + Benzo[e]pyrene + Perylene using HALO PAH
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Beer Fermentation Analysis using HALO Penta HILIC
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Analysis of Sugars in Pure Honey Using HALO Penta-HILIC Folder
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Analysis of Vet Drugs on HALO 1.5
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LCMS Analysis ADC Mimic HALO 1000A Phases
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Curmumin analysis inTumeric on HALO 1_5 C18
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291-E-Analysis of PFAS in Bottled Water by EPA method 1633
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290-E-EPA Method 1633 PFAS
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Enalapril Maleate According to Chinese Pharmacopeia (CP)
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Modified Ginseng Analysis According to Chinese Pharmacopoeia (CP) Method HALO C18, 2.7
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Analysis of Sucrose and Lactose in Chocolate Using HALO® 90 Å Penta-HILIC
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Sensitivity Increase of Mixed Proteins Through the Use of a 1.5 mm ID column
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Efficiency of 1.5 mm ID Columns Demonstrated Using Alkylphenones
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Increased Sensitivity in Small Molecule Applications with Cough & Cold Medications
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Increased Sensitivity of Intact Trastuzumab Using a 1.5 mm ID Column
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Increased Sensitivity and Solvent Savings of Trastuzumab Tryptic Digest using a 1.5 mm ID Column
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Sensitivity and Solvent Savings using a 1.5mm ID Column with Cannabinoids
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LC-MS Analysis of Veterinary Drugs Using HALO®C18
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Phase Comparison for Tocopherols and Tocotrienols
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LC-MS Analysis of Pesticides and Environmental Contaminants Spiked in Eggs using HALO® Biphenyl
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HPLC Separation of BTEX in Process Water
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LCMS of Pharmaceutical and Personal Care Products based on EPA 542
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PAH Stability at 600 bar
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LC-MS Analysis of Chantix® Varenicline NDSRI using HALO® Biphenyl
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Benzene Screening in Aerosol Sunscreens
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Fast Separation of Oligosaccharides using HALO 90 Å Penta-HILIC
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High Resolution Separation of Oligosaccharides on HALO 90 Å Penta-HILIC
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Comparison of an IdeS Digested mAb on Different HALO 1000 Å Phases
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Oxidation of NIST mAb Fragment
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Reduced IgG1 (Trastuzumab) Retention Comparison on Three HALO® 1000 Å Phases
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Cefotaxime Sodium According to Chinese Pharmacopeia (CP)
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Separation of Deamidation Products of the NIST mAb on HALO® ES-C18
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Capillary scale HILIC Separation of Deamidation Products of Trastuzumab
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Ginseng Analysis According to Chinese Pharmacopoeia (CP) Method using 5 µm HALO® C18
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Food Additives Assay using HALO® AQ-C18, 5µm
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Cefuroxime Sodium According to Chinese Pharmacopoeia (CP) Method
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Vitamin B12 Analysis According to Chinese Pharmacopoeia (CP) Method
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Dexamethasone Sodium Phosphate (EP 10.0)
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LCMS Screening Comparison of Mycotoxins in Craft and Home Brewed Beers
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LC-MS Separation of Mycotoxins on HALO® PFP, 2.7 µm
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Separation of Fat Soluble Vitamins Found in Multivitamins
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Separation of Water Soluble Vitamins Found in Multivitamins
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Chloroquine Phosphate Assay and Impurity Profiling
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Peptide Analysis of Bee Venom Assay for Antimicrobial Properties Using HALO Peptide
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Carotenoid Analysis in Pumpkin
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Large Panel Pesticide Screening on HALO Biphenyl
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Rapid Analysis of 33 PFAS Compounds in Under 5 Minutes
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Demonstration of HALO PFAS DELAY
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PFAS Analysis According to EPA 8327
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PFAS Analysis According to EPA 533
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PFAS Analysis According to EPA 537.1
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LCMS Separation of Barbiturates
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Separation of Explosives on HALO 2 C18
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HPLC Separation of Explosives on HALO column vs Competitor SPP Column
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PAH in Grilled Meat
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Separation of EU 15 + 1 using HALO PAH
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Sildenafil Citrate on HALO 5 C18
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Separation of PAH Compounds Using UV and Fluorescence Detection
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LCMS Separation of Bile Acids Using HALO® C18
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Separation of 18 PAH Compounds using HALO PAH
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Comparison of HALO PAH vs. FPP column for 18 PAH Compounds
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Separation of 16 PAH Compounds Specified in EPA 610 + 2 additional PAH Compounds using HALO PAH
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Glycan Lot to Lot Reproducibility
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Ginseng Analysis using 2.7µm HALO C18
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Ginseng Analysis Using 5 um HALO C18
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LCMS Separation of T3/rT3
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Isocratic Separation of 18 Cannabinoids
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Rapid Peptide Mapping of an Adalimumab Digest
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Ascorbic Acid Analysis Using HALO AQ-C18
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Rapid PFAS Analysis According to EPA 537.1 Using HALO 90 A Phenyl-Hexyl, 2.7 um
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PFAS Analysis According to EPA 537.1 Using HALO 90 A C18, 2 um
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Chocolate Analysis Using HALO 90 A Penta-HILIC
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Analysis of Sugars in Pure Honey using HALO Penta-HILIC
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Peptide and Protein Mix on HALO 400 Å ES-C18, 3.4 µm
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Separation of Phthalates and Neutral Compounds on HALO C8
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Separation of EU 15 + 1 Using HALO PAH
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PFAS Analysis of Well Water
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Enhanced Selectivity with HALO 160 Å Phenyl-Hexyl for a Tryptic Digest using LC-MS
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Analysis of Apotransferrin Tryptic Digest on HALO® 160 Å Columns
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Separation of Beta Blockers on HALO Biphenyl, 2.0 μm
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Isocratic Separation of 14 Cannabinoids on HALO® C18
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Isocratic Separation of Synthetic Cannabinoids Using MS Confirmation
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Separation of Fat Soluble Vitamins on HALO® C30
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High Throughput, High speed LC-MS/MS Separation of Mycotoxins on HALO® PFP, 2 μm
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LC-MS Separation of Corn Oil on HALO® C30 Compared to HALO® C18
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Pesticides Separation on HALO 90 Å Biphenyl
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Separation of Phenolic Acids on HALO® 90 Å RP-Amide, 2.0 μm
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Separation of 14 Cannabinoids on HALO® C18
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Fast Separation of Ten Cannabinoids on HALO® C18
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Isocratic Separation of Synthetic Cannabinoids on HALO® C18
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Higher Efficiency of HALO® C18 (2.0 μm Fused-Core®) Compared to a 1.7 μm Totally Porous C18 Column
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Polar Compounds Separated by HALO® RP-Amide, 5 μm
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Separation of Mixed Polarity Compounds on HALO® C18 and ES-CN
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Rapid HPLC Separation of Phenones on HALO® C18 Phase
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Comparable Selectivity Between HALO® 5 μm and HALO® 2.7 μm RP-Amide Phases
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Comparable Efficiency of HALO® Fused-Core® C18, 2.0 μm and Superficially Porous (SP) C18, 1.6 μm Columns
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Isocratic Separation of Anilines on HALO® C18
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Separation of Iodonium Salts on HALO® Phenyl-Hexyl
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Separation of Stilbenes on HALO® C8 and ES-CN, 5 μm
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Isocratic Separation of Phthalate Esters on HALO® RP-Amide
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Isocratic Separation of Phthalate Esters on HALO® C18
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Separation of Explosives on HALO® C18
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Separation of 17 Explosives on HALO® C18, 2.7 μm
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Isocratic Separation of Dinitrotoluenes on HALO® PFP Phase
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Separation of p-Hydroxybenzoic Acid Esters (Parabens) on HALO® C18, 2.7 μm
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Isocratic Separation of Dinitrotoluenes on HALO® RP-Amide Phase
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Separation of Aromatic Nitro Compounds on HALO® PFP and Phenyl-Hexyl
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Isocratic Separation of Anilines on HALO® RP-Amide
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Analysis of Vitamin A and Vitamin E Isomers using GB Method
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Separation of Water-Soluble Vitamins on HALO® AQ-C18
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Vitamin K1 Analysis: Temperature vs. Resolution
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Separation of Tocopherols on HALO® C30
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Vitamin K1 Isomer Analysis on HALO® C30
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Rapid Separation of Vitamin E Congeners on HALO® PFP
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Analysis of Curcumins on HALO® RP-Amide and HALO® C18
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Separation of Water Soluble Vitamins on HALO® HILIC, 2.0 μm
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HPLC Separation of Hesperidin and Diosmin on HALO® PFP, 5 μm
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Separation of Tocopherols on HALO® C30 based on GB (Chinese Standards)
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Separation of Resveratrols and Related Compounds on HALO® C18, 5 μm
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Separation of Melatonin and Related Compounds on HALO® RP-Amide
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Separation of Resveratrols on HALO® C18, 2.7 μm
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Separation of Biogenic Amines on HALO® Phenyl-Hexyl 5 μm by Ion-Pairing
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Separation of Diosmin and Hesperidin on HALO® Phenyl-Hexyl and HALO® RP-Amide
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Comparison of Selectivity of HALO® ES-CN, 5 μm and 2.7 μm Phases
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Separation of Neonicotinoids on HALO® C18, 2.7 μm
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Separation of Carbonyl Compound DNPH Derivatives on HALO® C18, 5 μm
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Separation of Carbonyl Compounds as Dinitrophenylhydrazone Derivatives on HALO® C18, 2.7 μm
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Comparable Selectivity Between HALO® 5 μm and HALO® 2.7 μm PFP Phases
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Separation of Neutral Aromatics on HALO® PFP, C18 and Phenyl-Hexyl
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LC-MS Analysis of Multiple Mycotoxins on HALO 90 Å Biphenyl
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Isocratic Separation of Aflatoxins on HALO® C18
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Rapid HPLC Separation of Aromatic Compounds on HALO® Phenyl-Hexyl
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Separation of Six Pyrethrins on HALO® AQ-C18, 2.7 μm
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Separation of Triazine Pesticides on HALO® AQ-C18, 2.7 μm
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Separation of Six Pyrethrins on HALO® C18, 5 μm
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Separation of Nonselective Herbicides on HALO® Phenyl-Hexyl, 5 μm
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Comparison of Separations on HALO® 5 μm Fused-Core® C18 and a Competitive 3.0 μm Totally Porous C18 Phase
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Separation of Phenyl Urea Pesticides on HALO® C18 Phase
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Separation of Phenyl Urea Pesticides on HALO® Phenyl-Hexyl Phase
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Rapid Separation of Triazine Pesticides on HALO® C18 Phase
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Separation of Carbamate Pesticides on HALO® C18 Phase
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Separation of Carbamate Pesticides on HALO® ES-CN Phase
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Separation of Capsaicins in Chili Powder on HALO® C18, 2.7 μm
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HPLC Analysis of Chlorogenic Acid in Green Coffee Extract on HALO® C18, 2.7 μm
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LC-MS Analysis of Stevia Extract on HALO® Penta-HILIC, 5 μm
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Separation of Food Additives on HALO® Phenyl-Hexyl and RP-Amide Phases
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Separation of Xanthines on HALO® RP-Amide Phase
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Separation of Vanillins on HALO® Phenyl-Hexyl Phase
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Separation of Vanillins on HALO® C18
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Separation of Polar Organic Acids on HALO® AQ-C18
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Separation of Phenolic Acids on HALO 90 Å RP-Amide, 2.7 μm
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Separation of Patulin and HMF on HALO 90 Å Biphenyl
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Separation of Hop Acids on HALO® 5 μm Biphenyl
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Separation of Five Flavonoids on HALO® C8, 2.0 μm
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Separation of Three Flavonoids on HALO® RP-Amide, ES-CN and Phenyl-Hexyl, 2.7 μm
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Separation of Three Flavonoids on HALO® RP-Amide, ES-CN and Phenyl-Hexyl, 2.7 μm
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Separation of Six Flavonoids on HALO® C18, 2.7 μm
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Separation of Carotenoids on HALO® C30
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Carotenoids Extracted from Carrot Juice Analyzed Using HALO® C30
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Determination of Caffeine in Soda Using HALO® C18, 5 μm
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LC-MS Separation of EtG/EtS from urine on HALO® Penta-HILIC, 2 μm
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LC-MS Separation SAMHSA 5 Panel on HALO® Biphenyl 2 μm
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LC-MS Separation of Kratom and its Metabolite on HALO® C18, 2 μm
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LC-MS Separation of Drugs of Abuse and Metabolites on HALO® Penta-HILIC
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Separation of Local Anesthetics on HALO® Penta-HILIC, 2.0 μm
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Separation of Glucocorticoids on HALO® C30
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Separation of Steroids on HALO 90 Å Biphenyl
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Separation of Steroid Hormones and Hormone Conjugates on HALO® C18
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Separation of Anabolic Steroids on HALO® C18, 2.0 μm
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Separation of Steroids on HALO® PFP, 2.0 μm
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Separation of Structurally Similar Steroids on HALO® C18 and PFP
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LC-MS Separation of Pain Management Opiates on HALO® Biphenyl, 2.0 μm
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Pain Management Panel Comparison on HALO® Biphenyl and C18
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LC-MS Separation of Fentanyl and Analogues in Synthetic Urine
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Comparable Selectivity Between HALO® 5 μm and HALO® 2.7 μm Phenyl-Hexyl Phases
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Separation of Benzodiazepines on HALO® PFP, 5 μm
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Separation of Benzodiazepines on HALO® Phenyl-Hexyl, C18, and PFP Phases
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Increased Resolution with HALO 400 Å Diphenyl Compared to FPP 300 Å Diphenyl
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Fast Peptide Separation with HALO 160 Å ES-C18, 2.0 μm
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Separation of Procainamide-Labeled Dextran Standards on HALO® Glycan
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Separation of PNGase-Released and Labeled N-Glycans by HILIC Using HALO® Glycan Column
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HPLC Separation of IgG2-B Monoclonal Antibody on HALO 400 Å C4, 3.4 μm
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Separation of Nucleotides on HALO® Penta-HILIC, 2.7 μm
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Analysis of Apotransferrin Tryptic Digest on HALO 160 Å ES-C18
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Separation of Nucleosides and Nucleobases on 2.7 μm HALO® Penta-HILIC
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HALO® AQ-C18 Separation of Nucleobases
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Effect of HALO® ES-C18 Pore Size on Protein Peak Shape and Width
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Protein Separation on HALO 1000 Å ES-C18, 2.7 μm
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Enhanced Selectivity for the Separation of Peptides Comparing HALO 160 Å with Three Different Bonded Phases
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Improved Separations with HALO 400 Å C4 Compared to Totally Porous C4
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HALO 160 Å ES-C18, 2.0 μm Lot Reproducibility
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High Temperature/Low pH Stability with HALO 160 Å ES-C18, 2.0 μm
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Very High Peak Capacity with HALO 160 Å ES-C18, 2.0 μm
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Effect of Silica Pore Size on Protein Separations
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Separation of Four Small Proteins on HALO® 160 Å ES-C18, 5 μm vs. Totally Porous C18, 3.0 μm
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Effect of Temperature on the Separation of Proteins on HALO 400 Å C4
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Separation of Seven Peptides on HALO® 5 μm 160 Å ES-C18 and ES-CN Phases
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Separation of Peptides and Small Proteins on HALO 160 Å ES-C18
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High Temperature/Low pH Stability with HALO 1000 Å ES-C18, 2.7 μm
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IgG2 Comparison on HALO 1000 Å C4, ES-C18, and Diphenyl
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LC-MS Analysis of Trastuzumab Using HALO® 1000 Å C4
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HALO 1000 Å C4 Protein Column for High Resolution Separation of a Monoclonal Antibody
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LC-MS Analysis of Reduced IgG1 Monoclonal Antibody Fragments Using HALO 400 Å C4
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Effect of Acid Modifiers on Intact mAb Peak Shape
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Analysis of Sunscreens using HALO® RP-Amide, 2.7 μm
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Paracetamol Impurities: European Pharmacopoeia 9.4 Method
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Amine Medications Separated Using HALO® C18, 5 μm
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Chinese Pharmacopeia Separation of Parabens on HALO® C18, 2.7 μm
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Separation of Polar Samples on HALO® AQ-C18 and C18
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HALO® C18, 5 μm Lot to Lot Reproducibility
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Comparable Selectivity of HALO® C18, 2.7 μm and HALO® C18, 5 μm
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Comparable Selectivity Between HALO® Penta-HILIC 5 μm and 2.7 μm
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Antihistamines on HALO® C18, 5 μm
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Sulfa Drugs on HALO® C18, 5 μm
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Separation of Xanthines on HALO® Phenyl-Hexyl Phase
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Separation of Statin Drugs on HALO® Phenyl-Hexyl in Acetonitrile
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Separation of Statin Drugs on HALO® Phenyl-Hexyl in Methanol
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Separation of Statin Drugs on HALO® C8
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Separation of Galantamine and Quetiapine on HALO® PFP
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Separation of NSAIDS on HALO® ES-CN, 2.0 μm with MS Compatible Mobile Phase
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Separation of NSAIDs on HALO® C18, 5.0 μm and Totally Porous C18, 5.0 μm
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Isocratic Separation of NSAIDs on HALO® ES-CN Phase
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Gradient Separation of NSAIDs on HALO® RP-Amide
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Separation of NSAIDs on HALO® C8
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Gradient Separation of NSAIDs on HALO® C8
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Isocratic Separation of NSAIDs on HALO® C18
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Rapid Isocratic Separation of Fibrates on HALO® C18 Phase
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Rapid Isocratic Separation of Fibrates on HALO® RP-Amide Phase
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Rapid Isocratic Separation of Fibrates on HALO® PFP Phase
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HPLC Separation of Diuretics on HALO® Phenyl-Hexyl
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Separation of Cephalosporins on HALO® Phenyl-Hexyl
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Separation of Cephalosporins on HALO® Penta-HILIC and HALO® HILIC
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Separation of Beta Blockers on HALO® Penta-HILIC, 2.0 μm
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Structures
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Separation of Beta Blockers on HALO Biphenyl, 2.0 μm
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Separation of Five Beta Blocker Drugs on HALO® Penta-HILIC
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Benzodiazepines Separation on HALO 90 Å Phenyl-Hexyl, 2.0 μm
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Separation of Paracetamol and Impurities According to EP 9.4
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Separation of OTC Common Cold Medicinal Compounds
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Comparable Selectivity Between HALO® HILIC, 5.0 μm and HALO® HILIC, 2.7 μm
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Isocratic Separation of Amphenicols on HALO^® RP-Amide Phase
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Isocratic Separation of Amphenicols on HALO^® Phenyl-Hexyl Phase
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Isocratic Separation of Basic Drugs on HALO^® PFP
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Separation of Antidepressants on HALO^® Penta-HILIC Stationary Phase
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Separation of Anticoagulants Using HALO^® 90 Å C18, 2.0 μm
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Rapid HPLC Separation of Anticoagulants on HALO^® Phenyl-Hexyl Phase
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Separation of Antibiotic and Antifungal Drugs on HALO^® RP-Amide
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Separation of Sulfonamides on HALO^® Biphenyl, 2.0 μm
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Amoxicillin and Ampicillin on HALO^® RP-Amide
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Separation of Penicillins on HALO^® Phenyl-Hexyl
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Separation of Penicillins on HALO^® ES-CN
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Separation of Cephalosporins on HALO^® ES-CN
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Separation of Fluoroquinolone Drugs on HALO^® Phenyl-Hexyl Phase
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Separation of Sulfa Drugs on HALO^® RP-Amide
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Separation of Antiulcer Drugs on HALO^® Penta-HILIC
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Rapid Separation of Sulfonyl Urea Drugs on HALO^® PFP Phase
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Rapid Isocratic Separation of Sulfonyl Urea Drugs on HALO^® Phenyl-Hexyl Phase
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Rapid Isocratic Separation of Sulfonyl Urea Drugs on HALO^® C18 Phase
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